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KMID : 0624620170500090472
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BMB Reports
2017 Volume.50 No. 9 p.472 ~ p.477
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Bach2 represses the AP-1-driven induction of interleukin-2 gene transcription in CD4+ T cells
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Jang Eun-Kyeong
Lee Hye-Rim
Lee Geon-Hee
Oh Ah-Reum
Cha Ji-Young
Igarashi Kazuhiko
Youn Jee-Hee
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Abstract
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The transcription repressor Bach2 has been proposed as a regulator of T cell quiescence, but the underlying mechanism is not fully understood. Given the importance of interleukin-2 in T cell activation, we investigated whether Bach2 is a component of the network of factors that regulates interleukin-2 expression. In primary and transformed CD4+ T cells, Bach2 overexpression counteracted T cell receptor/CD28- or PMA/ionomycin-driven induction of interleukin-2 expression, and silencing of Bach2 had the opposite effect. Luciferase and chromatin immunoprecipitation assays revealed that Bach2 binds to multiple Maf-recognition element-like sites on the interleukin-2 proximal promoter in a manner competitive with AP-1, and thereby represses AP-1-driven induction of interleukin-2 transcription. Thus, this study demonstrates that Bach2 is a direct repressor of the interleukin-2 gene in CD4+ T cells during the immediate early phase of AP-driven activation, thereby playing an important role in the maintenance of immune quiescence in the steady state.
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KEYWORD
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Bach2, CD4+ T cells, IL-2, Repressor
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